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A 25 μl qPCR consisting of 25 ng diluted cDNA, QuantiFast® Sybr Green Dye (Qiagen) and 0.1 μM of each qPCR primer pair was performed to obtain quantifiable expressions of Wnt and EMT-related gene targets in MCF7 and TamR cells.
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"Listen, classical music has always been a tough business, and the digital world is a quantifiable expression of new challenges.
Pathways are discussed to make biofilms more susceptible to antimicrobials by intervening with their viscoelasticity, as a quantifiable expression of their structure and composition.
In this paper we propose an alternative novel approach which takes into account quantifiable expression levels of all genes assayed.
As both the samples contained RNA extracted from tissues of approximately 35 mosquitoes each and pooled to form one single sample, a quantifiable estimation of gene expression was expected with a minimum chance of aberrations.
To do this, the authors took photovideomicroscopy recordings made from neonatal PER2::Luc SCN explants and applied image processing followed by a cluster and spectral cluster analysis, based on similarity of luminescence signals, to generate quantifiable maps of PER2 expression throughout entire SCN slices.
All of the 64 genes tested presented detectable quantifiable mRNA expression (Ct>35).
In the present study, we used the sensitive and quantifiable qPCR assay to examine the expression of H19 in ER+ and ER− breast tumor samples that contained at least 70% tumor cells and found that H19 expression was highly correlated with ERα expression in these tumors.
Based on previous studies, we used functional motor deficits measured by rotarod, CNS vascular permeability measured by FITC-albumin leakage, and astrocyte activation measured by GFAP expression as quantifiable traits predictive of susceptibility to PIFS [ 14, 15].
The levels of endogenous fluorescence would be a quantifiable measure of how much the expression levels are changes and this could provide an alternative measure to the qRT-PCR data.
All the genes had quantifiable mRNA levels by real-time quantitative RT PCR (Ct value <38), suggesting basal expression of this pathway in normal bladder (Table 2).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com