A 25 μl qPCR consisting of 25 ng diluted cDNA, QuantiFast® Sybr Green Dye (Qiagen) and 0.1 μM of each qPCR primer pair was performed to obtain quantifiable expressions of Wnt and EMT-related gene targets in MCF7 and TamR cells.
In this paper we propose an alternative novel approach which takes into account quantifiable expression levels of all genes assayed.
In the present study, we used the sensitive and quantifiable qPCR assay to examine the expression of H19 in ER+ and ER− breast tumor samples that contained at least 70% tumor cells and found that H19 expression was highly correlated with ERα expression in these tumors.
All the genes had quantifiable mRNA levels by real-time quantitative RT PCR (Ct value <38), suggesting basal expression of this pathway in normal bladder (Table 2).
In conclusion, we found that the absence of CD26 and other aberrant surface expression of T cell-associated markers allowed identification of a discrete, quantifiable population in nearly all MF/SS patients with PB tumor cells.
Within 24 hr after exposure to DEPs, quantifiable changes in cytokines, an influx of inflammatory cells and proteins, and up-regulation of gene expression of inflammatory mediators are observable in BALF from exposed rats (Rao et al. 2005).
As mentioned before, one method is the quantifiable, dual-colorimetric RNA-ISH assay developed by Yu et al. [ 29], which uses the expression of seven pooled epithelial and three mesenchymal transcripts.
As this might be due to the dynamic expression of Robo or to a lack of sensitivity of the staining method, we addressed this further in systems in which Robo levels are more quantifiable.
All of the 64 genes tested presented detectable quantifiable mRNA expression (Ct>35).
