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"Listen, classical music has always been a tough business, and the digital world is a quantifiable expression of new challenges.
In this paper we propose an alternative novel approach which takes into account quantifiable expression levels of all genes assayed.
Pathways are discussed to make biofilms more susceptible to antimicrobials by intervening with their viscoelasticity, as a quantifiable expression of their structure and composition.
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A 25 μl qPCR consisting of 25 ng diluted cDNA, QuantiFast® Sybr Green Dye (Qiagen) and 0.1 μM of each qPCR primer pair was performed to obtain quantifiable expressions of Wnt and EMT-related gene targets in MCF7 and TamR cells.
All of the 64 genes tested presented detectable quantifiable mRNA expression (Ct>35).
We confirmed that SYNβglu expression is quantifiable with ONP-glucopyranoside (not shown).
In order to explore quantifiable differences in gene expression between the groups of ASCs, a PCR array with genes known to play a role in obesity was utilized.
Based on previous studies, we used functional motor deficits measured by rotarod, CNS vascular permeability measured by FITC-albumin leakage, and astrocyte activation measured by GFAP expression as quantifiable traits predictive of susceptibility to PIFS [ 14, 15].
As both the samples contained RNA extracted from tissues of approximately 35 mosquitoes each and pooled to form one single sample, a quantifiable estimation of gene expression was expected with a minimum chance of aberrations.
To do this, the authors took photovideomicroscopy recordings made from neonatal PER2::Luc SCN explants and applied image processing followed by a cluster and spectral cluster analysis, based on similarity of luminescence signals, to generate quantifiable maps of PER2 expression throughout entire SCN slices.
However, a recent study [16] demonstrated that CD11c expression is quantifiable in whole blood and we therefore elected to test whole blood, as this sample type would be most readily translated to clinical benefit, whereas isolation of specific cell populations is currently impractical in a clinical setting.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com