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MLPA quality was ascertained by means of three different procedures.
Bone quality was ascertained clinically at the time of implant placement according to surgeon judgment.
The presence of a common support and the matching quality was ascertained.
Prior to further processing, RNA quality was ascertained by electropherograms on the Agilent 2100 Bioanalyzer.
Read quality was ascertained using FastQC [ 44] and the Bioconductor package MEDIPS (version 1.0.0) [ 45].
RNA quality was ascertained at each stage of processing acquisition, microdissection, amplification, fragmentation and studies were advanced only if optimal.
Similar(51)
RNA concentration and quality were ascertained by using the NanoDrop® ND-1000UV spectrophotometer and Agilent 2100 Bioanalyzer (microcapillary electrophoresis on RNA 6000 Nano LabChips, Agilent Technologies).
Quality control was ascertained by ensuring that the ratio (260 nm∶280 nm) of the absorbance of the extracted DNA to be 1.80 1.95.
The quality of extracted RNA was ascertained through gel electrophoresis. 1 µg of total RNA was subjected to reverse-transcription using oligo-dT and the ImProm-II™ Reverse Transcription System (Promega), following manufacturer's recommendations.
Quality of the RNA was ascertained by nanodrop spectrophotometry.
Quality of the oils was ascertained to be more than 98% pure.
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