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Over 240,000 high quality SAGE tags, corresponding to 16,629 annotated genes, provided a comprehensive survey of changes in the transcriptome in response to low temperature, from perception of the stress to acquisition of freezing tolerance.
SAGE libraries were developed from tissue collected at each of the five selected time points, sequencing of these libraries resulted in a total of 242,066 high quality SAGE tags (Table 1).
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And for W. Edwards Deming and J.M. Juran, the quality sages whose message Western car manufacturers scorned until the eleventh hour, read Watts Humphrey.
Two high-quality SAGE libraries containing a total of 76 854 tags were constructed.
We have constructed two high-quality SAGE libraries, TT 1 and TT 2, from the total mouse testis of adult B6 males (Table 1).
And it has a distinctive herbal quality – more sage, basil and fennel than sauvignon's cut grass – that, along with a generous freshening citrus, is subtly displayed in this version from Corsica (where the variety is spelled vermentinu), the kind of breezy dry white that cries out for grilled fish.
This animal model should help improve the overall quality of SAGE gene expression data.
This program provided criteria for assessing the quality of the SAGE libraries (length distribution of ditags, frequency of replicate ditags, and detection of linkers).
a. Tags remaining after the removal of duplicate ditags, ditags that fall outside of the 24 28 bp length and ditags possessing a low sequence quality read The SAGE output was restructured to exploit the advantages of utilising a model organism by referencing the results to annotated gene identifiers rather than individual tag sequences.
Based on his central thesis dealing with the inseparability of substance and function (Rošker 2009a: 377), Xiong created an ethical system rooted in the classical Confucian paradigm of the nobleman (junzi 君子), defined as someone who possesses the qualities of the "inner sage" and the "outer ruler".
The influence of many years spent in America talking to (and often down to) Americans also gave his performance a kind of Barnum quality: Hitchens the cracker-barrel sage selling snake oil dressed up as urgent verity.
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Justyna Jupowicz-Kozak
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