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Field measurements of pH and EC were made using a handheld multi water quality probe (HANNA HI 9828, USA).
Water column pH, dissolved oxygen (DO), nitrate (NO 3 ‒ ), and ammonium (NH4 +) were measured in situ using a YSI 6920 multi-parameter water quality probe (YSI Inc., Ohio, USA).
He spent two months in a canoe, dragging a water quality probe that collected data at five minute intervals.
The normalization and data filtering resulted in 1088 high quality probe signals, which represented both human and porcine miRNAs.
At the end of each trial, the carbon dioxide (CO2) concentration in the two arms of the Y-olfactometer was quantified using a 435-4 Testo multi-functional meter equipped with an indoor air quality probe [range: 0 to +10000 ppm CO2, accuracy: ±50 ppm CO2±2% of mv, 0 to +5000 ppm CO2] and an axillary measure of volunteer temperature was assessed.
Present day microarrays are vastly superior to their predecessors in terms of quality, probe density, and they can represent an entire species genome [ 2- 4].
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Many Coast Salish tribes have begun attaching water quality probes called YSI Sondes to their boats during the month-long Canoe Journey.
When choosing a small minimum inter-probe distance (≪probe-length) for the intergenic regions a "selective tiling" is achieved, i.e. high density, but with high quality probes only (see Table S1 for maximum probe density).
From the 411 probes with normal distribution in both sets as well as in the combined set, 131 failed the range criterion leaving 280 high quality probes in 250 genes for further study of heritability, age and gender effects.
The custom MBNI CDF-file (MmNuGOMm1a520177 version 9.0.1), available at http://brainarray.mbni.med.umich.edu/Brainarray/Database/CustomCDF/CDF_download_v9.asp and http://nugo-r.bioinformatics.nl/NuGO_R.html [79], [80] was used to re-annotate the probes to new probesets, remove poor quality probes and derive unique signal values for different probesets representing the same gene.
The next step was filtering probesets by flags to remove poor quality probes (absent flags).
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CEO of Professional Science Editing for Scientists @ prosciediting.com