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Good and fair quality oocytes produced a higher cleavage rate (P<0.01) than poor quality oocytes.
Embryo development with poor quality oocytes was arrested at the two to sixteen cell stage.
Morula production rate was also higher (P<0.01) for good as compared with fair quality oocytes.
However there was no decrease recorded in the meiotic division resumption rate and the proportion of good quality oocytes.
A critical stage in the optimization of in vitro maturation (IVM) is the selection of good quality oocytes.
In experiment 2, good quality oocytes were cultured for maturation in: (1) CR1aa; (2) CR2aa; (3) TCM-199; (4) MEM or (5) RPMI-1640, and then fertilized using frozen thawed buffalo spermatozoa in CR1aa.
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Currently, there is no uniformly accepted definition of decreased ovarian reserve (DOR), as the term may refer to three related but distinctly different outcomes: oocyte quality, oocyte quantity, or reproductive potential.
In addition to the morphological criteria, several molecular and biochemical (from metabolomics) markers have been described helping to define a "good quality" oocyte.
Significant correlations were observed in the luteal phase between the total number of oocytes and cholesterol (Cho), HDL, sodium (Na), chloride (Cl); A-quality oocytes and Na, Cl, Mg; C-quality oocytes and Cho, HDL, and Mg in follicular fluid.
In a preliminary, unpublished randomized study conducted in 2000 on 39 patients, including a placebo group, it was observed that the addition of growth hormone (GH) during ovarian stimulation in patients with poor-quality oocytes increased the pregnancy rate.
Furthermore, no significant difference was observed between the groups in the number of good-quality oocytes, leading follicles, good-quality embryos, or in fertilization, implantation, positive beta-HCG and clinical pregnancy rates.
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