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Reads with a mapping quality of zero were discarded.
When a short read can be aligned to more than one position in the genome with the same mapping quality, this read is dropped, being assigned a mapping quality of zero.
This is due to reads that mapped into low-complexity or repetitive DNA (yielding a BWA map quality of zero), reads that required excessive gaps or mismatches in their alignments to the reference, and reads that mapped to loci that received support from fewer than 10 read pairs across all experiment (see Methods).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com