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The quality of the draft genome (assembled contigs) was assessed through blastx searches against known L. edodes genes available in protein databases (336 proteins).
There was some debate in the House of Lords, with questions and amendments covering Section 1, but the bill was not substantially amended, something North puts down to the quality of the draft prepared by the Law Commission.
Another concern is the quality of the draft genome (454 sequencing) data.
The quality of the draft genomes was measured by coverage of Roche 454 reads and Illumina reads.
Since the five species considered are closely related, this can be due to the quality of the draft sequence.
Coverage and quality of the draft genome sequence were assessed by reference mapping of trimmed paired-end reads using CLC Genomics Workbench.
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An additional 528-primer walk reads were created as needed to address low quality regions of the draft assembly.
These genome alignments also served to visualize and quantify the overall quality of the individual draft assemblies for the test strains.
Once this decision is taken, a genome project requires careful planning with respect to the organism involved and the intended quality of the genome draft.
Semi-structured interviews and focus groups were selected as complimentary qualitative methods to assess the feasibility of the iCST programme, and the quality of the first draft of the materials produced.
As predicted by Niimura and Nei [ 13], the quality of the second draft of the chicken genome increased and the number of short contigs that are few kilobases long decreased.
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