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This control sample was used to assess the extent of overexpression and quality of labeling for a given experiment.
In terms of the quality of labeling, the cells were consistently labeled in decreasing numbers as the survival time increased with both analogues, as would be expected.
We found no difference in the quality of labeling between the axons in BD animals and those in animals with normal vision.
To determine whether such doses were appropriate to achieve the quality of labeling required in the experiments, we previously carried out a series of experiments in which both thymidine analogues were injected into the same animal and the cells were labeled after different survival times in different groups of animals.
The quality of labeling using both iTRAQ and succinic anhydride was determined by the disappearance of signals from unlabeled analytes in the mass spectra.
Unlike other previously reported combinations of anterograde and retrograde agents, the present approach enables the exploitation of two vectors with the same diffusion characteristics, high quality of labeling and short time course for expression as shown in Fig. 1d.
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The quality of labeled and fragmented cRNA was analyzed using the Agilent bioanalyzer 2100.
The amount (15 52 µg) and quality of labeled cRNA were measured using both the NanoDrop spectrophotometer and Agilent 2100 Bioanalyzer.
The quality of labelled cRNA target was assessed by hybridization to Affymetrix test II arrays.
Thus, this marker enzyme provides superb detection quality of labeled cells in the total absence of background staining.
The quality of labeled cRNA was evaluated on the Agilent Bioanalyser 2100 and quantified using an ND-1000 NanoDrop spectrophotometer.
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