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These strategies were an effective way to improve the quality of assembly and annotation of assembled unigenes [ 23].
Over 83 % reads could be mapped back to the assembled transcripts, indicating a high quality of assembly.
Over 80% reads could be mapped back to the assembled transcripts, indicating a high quality of assembly (Additional file 1).
In order to ensure the quality of assembly, the reads were mapped back to the assembled transcripts using the bowtie aligner by Visualization and Quality Assessment application within Trinity software.
Moreover, a final step that maps all reads back to the assembled transcripts can also help to improve the quality of assembly.
Among other production bottlenecks, drilling-induced delamination remains a major defect which adversely affects the quality of assembly parts.
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Though Minia is classified as a low-quality assembler, the quality of assemblies for Minia gets enhanced as the expected genome size increases.
A model based monitoring method is developed with systematically collected experimental data and fundamental process knowledge to verify the quality of assemblies.
The quality of assemblies generated was assessed using the perl scripts given in the NGS QC Toolkit.
There have been several efforts to assess the quality of assemblies produced by de novo methods.
We also examined the effect of coverage depth, hash length, alternative splicing and assembly merging on the quality of assemblies.
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