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Then we used Trimmomatic (v0.32) (Bolger et al. 2014) software to trim low quality ends of all reads.
As long as it takes a few cues on the software/image quality ends as well, that's perfectly okay with us.
It appears that the automated assembly was conservative in trimming reads for low quality ends, and in not extending contigs beyond apparent length differences in the constituent reads.
As previously reported [18], we cloned a total of 4,032 cDNAs from the two SSH libraries, sequenced 3,816 positive clones, and trimmed off contamination of low quality ends and vectors/adaptors using CodonCode Aligner.
In order to minimize the mining of technical errors we defined a stringent threshold for alignment quality (expect≤1E-21) and the low quality ends of alignments were discarded.
Low quality ends were removed from the consensus contig sequences.
Similar(34)
"The Liverpool Care Pathway was originally devised to help doctors and nurses provide quality end-of-life care.
"The quality ended up not so great, but a lot of it was our fault," Ms. Eng said.
The firm has positioned itself at the quality end of the market, where ethically-minded consumers are concentrated.
Hospices, which provide high quality end of life care, accommodate just 6 per cent of those who die each year.
While it would be wrong to say that the island has been rebranded, there has certainly been a concentration on the quality end of the market.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com