Myth: Gem-quality cultured diamonds are easy to grow.
In the development of a mass production system for iPS cells, it is desirable that a uniform quality of cultured cells is maintained for a long-term.
Mycoplasma hyorhinis infection has an important role on the quality of cultured human colon cancer cell lines giving a false positive increase of cancer stem cells fraction characterized by CD133 expression.
It is widely assumed that low TEER indicates low quality of cultured epithelial cells; however, here we clearly demonstrate that excessive transepithelial resistance in cells originating from a leaky epithelium is associated with a sub-differentiated culture status.
For the present study, our laboratory received a total of 105 poor-quality embryos cultured in a modified medium.
Size is the most important and valuable quality of the cultured black-lip pearl, Pinctada margaritifera.
In experiment 2, good quality oocytes were cultured for maturation in: (1) CR1aa; (2) CR2aa; (3) TCM-199; (4) MEM or (5) RPMI-1640, and then fertilized using frozen thawed buffalo spermatozoa in CR1aa.
Although the reduction in telomere length and telomerase activity may not be the major cause of the senescence, they could provide a useful information for the quality of the cultured epidermis.
Another issue is that wide therapeutic use will require improvement in the uniformity in the quality of the cultured cells.
Quality assurance of cultured cell lines, in terms of their authentication, stability and contamination are dealt with above (see Section 1).
