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Multiple control probes were included on each chip for quality controls, sample labelling and assay conditions.
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Black spheres represent the quality control sample used to verify the reproducibility and accuracy of the data.
The first is a regular quality control sample to monitor the LC-MS response in time.
vRNA can be used as a quality control sample.
For some batches, we replaced the interbatch quality control sample with the SRM 2585 dust sample.
One reagent blank and one quality control sample were analyzed simultaneously with each batch of samples.
In comparison the coefficient of variation for a quality control sample was less than 15%.
A quality control sample was included in each run to verify proper operating conditions were achieved.
The method was validated by evaluating the quality control sample and recovery.
Each 96 well plate included one inter-plate positive quality control sample (NA06990 Coriell Cell Repositories).
Laboratory personnel were blinded to subject and quality control sample identity.
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