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In short, we applied the standard quality control pipeline for subjects (genotype success rate >95%, genotype-derived gender concordant with reported gender, excess inter/intra-heterozygosity) and for SNPs (Hardy-Weinberg Equilibrium p>1×10−6; Minor Allele Frequency >0.01, genotype call rate >0.95).
A step by step quality control pipeline was performed [ 20].
One dataset that had no information but passed the quality control pipeline was also included.
bnumber of sequences remaining after the data quality control pipeline treatment applied on raw reads.
Further quality control is performed using the ArrayAnalysis Quality Control pipeline (www.arrayanalysis.org/).org/
The raw Illumina reads were pre-processed using our in-house quality control pipeline.
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All data submitted to the diXa warehouse are subjected to quality control using Genedata Expressionist® (https://www.genedata.com/products/expressionist/) quality control pipelines.
An evaluation of quality control pipelines for each platform, like the one conducted for NGS data in the SEQC study, can guide harmonization of quality control by identifying best practices.
Quality control pipelines are also of considerable importance, and there is an urgent need to define appropriate quality control steps for single-cell analysis to ensure the integrity of data sets.
We subjected the raw recombination data to a comprehensive quality-control pipeline to detect errors and to test expectations regarding the ratio at which different types of events should be observed according to Mendelian rules (File S7 and Table 2).
Fluidized dense-phase pneumatic conveying of powders is becoming increasingly popular in various industries, such as power, chemical, cement, refinery, alumina, pharmaceutical, limestone, to list a few, due to the reasons of reduced gas flows and power consumption, improved product quality control, reduced pipeline sizing and wear rate, increased workplace safety etc.
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