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We define a distance metric between samples based on seven sequencing quality control metrics from Picard (http://broadinstitute.github.io/picard).github.io/picard
Our CNV calling pipeline (discussed further in the Supplementary Material) works as follows: We define a distance metric between samples based on seven sequencing quality control metrics from Picard (http://broadinstitute.github.io/picard ).github.io/picard
Comparing quality control metrics across all cells is helpful in identifying outliers.
Quality control metrics should be calculated for raw reads, as well as for aligned reads.
QC3 monitors quality control metrics at each stage of NGS data and provides unique and independent evaluations of the data quality from different perspectives.
Finally, the chapter discusses genomic test development in clinical oncology: technical issues, data analysis, quality control metrics, test validation, and results interpretation and reporting.
Greater than 80% of the data passed both quality control metrics.
Preliminary analysis of the scanned data including monitoring of quality control metrics was performed using the Illumina BeadStudio software.
Principle Component Analysis of all quality control metrics following MAS5.0 feature extraction was carried out (Figure 8 A).
Mean array quality control metrics were assessed for all 64 vascular endothelial biopsy samples hybridised to GeneChips.
A variety of quality control metrics ensured high reproducibility and quality of the ChIP-chip data (see Materials and methods, Text S1, and Fig. S3).
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