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Each reaction for each plate was filtered by using the pass fail quality control metric (Fluidigm) and normalized against Actb.
The first quality control metric is the mad1.dr score calculated by the segMNT algorithm in NimbleScan 2.6.
The mean standardized uptake value (SUV) of normal liver, not involved with cancer, was calculated and recorded and used as an internal quality control metric.
Prior to statistical analysis, the scans were inspected using the Agilent Quality Control report as well as an internal quality control metric including spike-in RNA controls.
The image files were then opened in Agilent's Feature Extraction software using the default Gene Expression protocol and the GE1_QCM_Feb07 quality control metric set.
In addition, a novel method to estimate the genotyping error rate is implemented in OpenADAM, which may serve as a data quality control metric and an aid in the calculation of statistical power.
Similar(53)
We define a distance metric between samples based on seven sequencing quality control metrics from Picard (http://broadinstitute.github.io/picard).github.io/picard
Our CNV calling pipeline (discussed further in the Supplementary Material) works as follows: We define a distance metric between samples based on seven sequencing quality control metrics from Picard (http://broadinstitute.github.io/picard ).github.io/picard
The tracking database stored information relating to the quality control (QC) metric of the chip hybridization and washing process, including border feature variation, chip to chip variation, and variation among standard controls distributed within each chip.
As usual in literature, and not withstanding the well-known limitations, the PSNR will be used here as the quality metric for quality control.
She presented a novel "stress" metric for quality control, which quantified the extent of changes to raw expression values during normalization and identified samples with unusually compressed or skewed distributions of raw intensity measurements.
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