Random 5' sequencing of cDNAs from developing fruits generated a total of 1273 ESTs, resulting in 942 sequences (74%) passing quality check (see Table 1).
After quality check, 230 high-quality microarrays (Table S1) were chosen for downstream analyses.
Identity rates between the designed sequences and the initial, native sequence are commonly used as a first quality check for CPD, and are given in Table 1.
After a quality check, 5494 high-quality ESTs were obtained (Table 1).
As shown in Additional file 3: Table S2, after a stringent quality check and data cleaning, all PE reads were assembled into 103,517 unigenes.
In the Quality Check section RAP organizes results obtained from both FastQC and NGS QC Toolkit in a comprehensive summary table.
After raw reads filtering and quality check, about 54 millions of 90 bp reads were obtained with 52.94% GC percentage (Additional file 1: Table S1).
After a stringent quality check and data cleaning, 53,437,444 paired-end reads with a total of 4,007,808,300 bp were obtained and assembled into 57,050 unigenes (Table 1).
(2) Quality check of CDR3-containing readseads.
We obtained allelic genotype calls for loci for which both the E and G reactions passed quality checks (Additional file 4: Table S2).
