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The quality and quantification of the RNA was checked and estimated by agarose gel electrophoresis and spectrophotometric analysis.
Total RNA was purified from each tissue, with assessment of RNA quality and quantification of purified RNA performed according to methods described previously [ 29].
However, before comparing mRNA expression profiles across samples, correction for variables such as quality and quantification of the starting material and enzymatic efficiencies must be carried out [ 26- 28].
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For quality check and quantification of the RNA-seq libraries, we employed 2100 Bioanalyzer and DNA 7500 kit (Agilent).
An introduction to the engineering science of water quality characterization and quantification of treatment objectives.
This chapter is an introduction to the engineering science of dirty water flow and quality characterization and quantification of treatment objectives for various end uses.
Quality control and quantification of DNA were performed before and after bisulphite conversion.
Quality assessment and quantification of cRNAs were performed with Agilent RNA kits on Bioanalyzer 2100.
Quality assessment and quantification of cRNAs were performed on Bioanalyzer 2100.
Quality control and quantification of library was performed with a Bioanalyzer chip DNA 1200 series II (Agilent Technologies, USA).
Quality control and quantification of the libraries were performed using a DNA 1000 series II Bioanalyzer Chip (Agilent).
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