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The atmosphere at the Proms is absolutely unique, thanks to the quality and concentration of the audience.
In December, the world's largest investigation into this area found quality and concentration had fallen sharply.
PCR and a Bioanalyzer were used to check the RNA for DNA contamination, quality, and concentration.
The RNA quality and concentration was determined by Nanodrop (ISOGEN Life science, De Meern, The Netherlands).
The quality and concentration of the extracted DNA were determined with a Nanodrop 2000 spectrophotometer (Thermoscientific).
The quality and concentration of extracted RNA samples were assessed spectrophotometrically using a NanoDrop (NanoDrop 2000, Germany).
RNA quality and concentration were measured by agarose gel electrophoresis and NanoDrop 2000/2000C (Thermo Scientific, USA).
The quality and concentration of DNA was examined by 1% agarose gel electrophoresis and spectrophotometer analysis (NanoDrop 1000, Thermo Scientific).
The quality and concentration of RNA were determined by agarose gel electrophoresis and spectrophotometric analysis (Eppendorf, Germany).
RNA quality and concentration was measured using Agilent's RNA 6000 Pico kit and 2100 Bioanalyzer machine.
The DNA was subsequently run on agarose gel to verify its quality and concentration.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com