While these approaches generally produce high-quality affinity reagents, they don't fully meet the need for rapid development, low cost, and application-specific flexibility.
Briefly, interactions were assessed using a peptide microarray followed by confirmation of positives and measurement of binding affinities by fluorescence polarization (FP), a high-quality affinity measurement method (Stiffler et al., 2007).
So be very selective and forgo numbers for quality and affinity.
Triad Therapeutics' proprietary technologies enable dramatic increases in both the speed of lead compound generation and the quality (binding affinity and specificity) of the compounds produced.
Their aesthetic refinement was characterized by, among other qualities, an affinity for meticulous rendition.
Recently, a 2 year effort mapping structural models to high-quality binding affinity data of ZF/DNA complexes (Temiz and Camacho, 2009) led to the development of a novel interaction code.
The six windows (c. 1475 1500) of the church of St. Gommaire at Lier and the Virgin window (c. 1482) at Anderlecht, although restored, have original portions of excellent quality and close affinity with contemporary Flemish painting.
This does not exclude antibodies for being the mediator of protection as it may not be the magnitude but rather the quality (such as affinity) or the fine specificity of the humoral response.
The data quality precluded evaluating affinity constants for the weaker interactions, but sulfate and selenate were measured at 34.4 and 23.8 M–1, respectively.
The quality of these affinity measurements was assessed by the internal consistency test, which had numerical values of 0.56 (3 hr elution measurement) and 0.626 (4 hr elution measurement) when these values have to be inferior to 3 for the measurements to be valid.
