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At constant STRESS, the likelihood of proliferation will decrease as proBias is increased, and at constant proBias increasing STRESS causes qualified CELLS to be less likely to proliferate.
Some potential obstacles to applying the reverse genetics approach include the need for qualified cells for virus production and intellectual property for this technique.
This technique allows infectious virus to be recovered from cells approved for use in human vaccine development (so-called qualified cells).
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The cells qualified for cell line establishment were stored in liquid nitrogen for subsequent analysis.
Studies on integrin receptors presented evidence that beta1 integrin expression by colon carcinoma cells qualifies these cells to successfully adhere to secondary sites.
The putative SMs were filtered based on the following criteria: (1) homozygous normal in all normal cells (at least read-covered in six normal cells); (2) the homozygous genotypes in normal cells were consistent in normal tissue; (3) at least mutant in three cancer cells among a total of 44 qualified cancer cells.
Compared to traditional viral methods, the aforementioned approaches can be used to generate qualified iPS cells (Table 1) without the risk of insertional mutagenesis.
We achieved an average of 88.6% whole-exome coverage of all qualified single cells (Additional file 2: Table S1) and covered more than 60% of the target region greater than 5× sequencing depth in all cells (Additional file 3: Figure S2C-D).
This implies that the majority of the CD69+ and CD69−CD8+ T cells of BM are not senescent cells but rather qualify as cells preserving long-term memory [ 28, 29].
However, considering our findings in the rodent experiments which showed therapeutic GLP-1 effects after ventricular encapsulated cell biodelivery, GLP-1 might be a prototype for a substance qualified for cell-based delivery into the CSF for the following reasons: - Its small molecular weight facilitates diffusional and convective distribution through the brain tissue.
(3) P (i ) = C n i p f i 1 − p f n − i P (i ) · S < 1 i the cancer cell number of mutant of a specific mutation n the total number of <span class="lh">qualified cancer cell p f the FDR P i) the probability under binomial distribution S the whole-exome size Then the criterion (3) was set according to the largest cell number fulfills above inequation.
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