Down selection using an extended set of discovery samples and qualification samples confirmed 23 MFs that consistently changed in abundance between baseline and 1, 2 and 6 months of therapy, with 12 MFs achieving statistical significance (p < 0.05).
For each study, Table 3 reports the rater qualification, the sample description, the description of the materials and setting, the classification procedure (re-scoring), the procedures chosen to describe inter-rater reliability, and the observed inter-rater reliability by correlation, mean score differences, and kappa.
To assess samples in a manner that more closely resembles what might occur during a clinical trial, the Qualification Set samples were not subjected to γ-irradiation; a process that was applied to the other samples and resulted in elevated temperatures and potential alteration of some metabolites.
It is noted that the Expanded Discovery Set-1 includes the D0 and M1 samples used in the initial TB-ETRB discovery phase, whereas the Qualification Set samples were analyzed for the first time during the down selection and qualification phase.
During the QC steps, Agilent 2100 Bioanaylzer and ABI Step One Plus Real-Time PCR System are used in quantification and qualification of the sample library.
Continued study of tumor heterogeneity and its impact on PD-L1 expression will aid in development of appropriate sample qualification criteria for PD-L1 IHC assays.
During the quality control steps, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System were used in quantification and qualification of the sample library.
During the QC steps, an Agilent 2100 Bioanaylzer and an ABI StepOnePlus Real-Time PCR System were used in quantification and qualification of the sample library.
During the quality control steps, Agilent 2100 Bioanaylzer (Agilent Technologies, Palo Alto, CA, USA) and ABI StepOnePlus Real-Time PCR System (ABI, New York, NY, USA) were used for quantification and qualification of the sample library.
During the QC steps, an Agilent 2100 Bioanalyzer and an ABI StepOnePlus Real-Time PCR System were used for quantification and qualification of the sample library.
