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The sequencing data were subjected to a strict QC test before bioinformatics analysis.
Quality control activities may range in complexity from relatively simple test with TLC, which is often the main QC test required in case of Tc-99m labelled kits, to a full series of QC tests, including HPLC, TLC, GC, gamma spectrometry, etc., in case of PET radiopharmaceutical preparations.
Finally, a QC test was adopted to check the reliability and repeatability of different cultures.
All the cultures assessed passed this QC test (Table 1) and were suitable for use in subsequent experiments.
MIC data were used only if the daily QC test results were within ranges considered acceptable by CLSI [ 13].
One sample (Q7 cells treated with Boc-DON) failed the QC test and was excluded from further analysis.
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Daily QC testing was performed using QC strains Enterococcus faecalis ATCC 29212, S. aureus ATCC 29213, S. pneumoniae ATCC 49619, E. coli ATCC 25922, P. aeruginosa ATCC 27853 and H. influenzae ATCC 49247 and ATCC 49766, as appropriate.
As described in FPC test (see Supplemental Table 2), a median of 2.40 × 10 cells (range 1.35 × 10 3.73 × 10) was collected to perform QC tests for batch release whereas the median amount of cells frozen for prospective QC testing was 0.96 × 10 (range 0.54 × 10 5.58 × 10).
In Japan, radiographers perform all QC testing, whereas in Finland, Iceland and Hungary the service engineers tend to be in charge of the QC tasks.
To investigate the influence of geography on quality control (QC) testing in the point of care testing (PoCT) in general practice trial.
The results of this validation study demonstrate that the 2D-LC method is accurate, sensitive, and robust and is ultimately suitable for QC testing with good method transferability.
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