The grids were put successively through PBS, 1% glutaraldehyde in PBS (10 min), PBS, PBS glycine, 2% BSA-TBST.
This movement takes place successively and cyclically.
With the development of HVDC technologies, Europe, the United States and China have successively put forward a plan for using multi-terminal HVDC systems (multi-terminal HVDC, MTDC) and DC grids [7, 8] for the integration of renewable energy.
The normal braking system of freight trains is based on depression of a single brake pipe and its slow propagation towards the end of the train, successively putting all distributors into service when they detect the pressure drop.
Samples taken from the scaffold were fixed in glutaraldehyde (Sigma) overnight and fixed with 1% osmic acid and then were dehydrated in 30%, 50%, 70%, and 90% alcohols successively and put in a critical point dryer for 10 min.
The random load histories are generated stepwise by putting together series of load cycles, successively predicted by the modeller.
A modification of this approach involves "multiple shooting," where several Poincaré sections are put in phase space, and one successively integrates from one to the next and concatenates the results [59, 75].
Such an amendment would have to be approved by two successively elected Legislatures and then put to voters.
Reading them in immediate sequence put me in mind of a projector flicking successively through photo stills to form a moving picture.
From the unacceptable level of debt we inherited - debt at 44percentnt of national income - I forecast debt in the coming year will fall to 30.3percentnt and in the following years I project 29.6, 29.7, 29.9 and 30percentnt successively - meeting our second rule and putting Britain in a far stronger position to deal with the ups and downs of the economic cycle.
