By in vivo covisualization of oskar mRNA with Staufen, its putative trafficking protein, we find oskar mRNA to be present in particles distinct from Staufen for part of its transport.
Based on the predicted topology of UNC93B1 (Brinkmann et al., 2007), the N- and C-termini face the cytosol, so we reasoned that these regions would be most likely to interact with putative trafficking factors.
The resulting set of sequences was searched for putative vesicle trafficking components.
Cell-to-cell transfer of lactate cannot be directly assessed in vivo, and transporter inhibition or knockdown may be used to assess putative lactate trafficking.
In the present study, we have sought to determine the nature of these putative processing or trafficking pathways of PrPd in different sheep scrapie sources.
We find that DE-cadherin, the transmembrane protein of adherens junctions, is actively trafficked through putative vesicles, and appears to travel through both Rab5-positive and Rab11-positive structures.
This protein is known to appear as cytoplasmic vesicular structures with putative role in vesicular trafficking (Hayashi et al., 2001).
The 129 repressed genes showed putative roles in vesicle trafficking, signal transduction, nitrogen metabolism, or molecular transport.
In Lactococcus lactis, which lacks the heme biosynthetic pathway, the HemN homolog named HemW is not a functional Coprox but a heme-binding protein with a putative role in heme trafficking (Abicht et al. 2012).
Indeed, this putative effect on protein trafficking could also mediate effects of Vangl2Lp through PCP-independent pathways, as suggested by the finding that Vangl2 regulates the cell-surface availability and levels of MMP14 in migrating cells during gastrulation (Williams et al., 2012).
