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In addition, seven novel putative miRNAs were predicted from a limited number of ESTs.
Over 60% of the putative miRNAs were detected in more than a single sample.
The potential targets of these putative miRNAs were also predicted based on sequence homology search.
We used Srnaloop to predict putative miRNAs from the silkworm genome.
Expression of putative miRNAs from sparse hotspots was confirmed by Northern blot analysis (Figure 3b).
Putative miRNAs.
The remaining sequences were considered putative miRNAs.
We also predicted the target genes for 384 putative miRNAs.
These putative miRNAs were excluded from further analysis.
MiRDeep2 flagged four putative miRNAs as possible tRNA/rRNA.
We validated the 4 putative miRNAs and further characterised one of them, mm_br_e15_1181, throughout embryogenesis.
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