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The paucity of information related to morphology, pluripotency markers, differentiation capability hampers a thorough evaluation of the validity of putative lines.
Two of the putative LINEs are located inside the indels and only found in Spirit's haplotype.
In Figure 5b, when we sum up the frequencies for every 20 bases, there is an additional peak at around 6 k which most likely corresponds to putative LINEs.
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Trials 2 and 3 were designed to test whether the putative line difference in stress response would impact on physical meat quality.
A wallaby LINE-1 segment [GenBank: DQ275763] was used to search for putative LINE segments.
The mechanisms underlying the generation of these CNVs in the devil MHC are not clear, though a possible explanation may be implied from the presence of the putative LINE fragments in the affected regions encompassing the three CNVs and Saha-UA, UB and UC genes.
Therefore, we decided to use the putative homozygous lines as the pure lines for the LSE2 and LSE3 mutations to characterize lse2 and lse3 plants, respectively.
This is the first study to establish and characterize the iPSC-derived putative ESC lines and compare them with other lines derived from different origins in pigs.
Shown in Figure 14 is a PCR confirmation of the genotype of putative H/H lines # 6-3-10-19, # 6-3-20-1 compared to WT, AS11 and lpcat2 lines.
Analysis of all putative transgenic lines using PCR established the presence of the nptII and cry9Aa2 genes in all regenerated lines.
Five putative transgenic lines were selected and examined for the presence of FLC.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com