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Such a prohibitive number of putative interactions make the interactive exploration of the data extremely difficult.
Based on a reverse genetic analysis of the DENV-2 MTase and the RdRp domains, complemented by in silico docking approach to map their putative interactions, a model for the full-length WNV NS5 protein was put forward [36].
Putative interactions are tested rigorously for replication and for biological plausibility.
Compounds exhibiting good antioxidant activity were docked with tyrosinase enzyme (PDB ID: 3NM8) from Bacillus megaterium to predict their putative interactions.
To solve this problem by rational structure optimization, a short series of C18-deoxy analogues of herbimycin A were designed based on putative interactions between the compound and the protein.
Analysis of the putative interactions between AcpS and ACPM from Mtb, based on a comparison with the complex structure from Bacillus subtilis, showed that the Mtb AcpS and ACPM lack the electrostatic complementarity observed in B. subtilis.
Those novel interactions represent putative interactions which are not yet experimentally proven.
A range of new technology platforms and bioinformatic tools now exist with which to test hypotheses around these putative interactions.
Forty-five of these 79 putative interactions were also identified in human cells by co-immunoprecipitation (co-IP) experiments.
However, the distribution of putative interactions detected by 4Cv is non-random and similar to those observed with previously validated 4C techniques [23], [29].
A first group of mutations were designed in order to disrupt putative interactions between the agonist and the binding pocket (Figure S3).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com