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The IACS causally excises most of the active region of space-time (the ergosphere) that would be the putative element to enforce rapid rotation of the system.
One of these regions contains a sequence homologous to a putative element, N30, previously identified in a homology search of promoter regions in a range of animal species [18] (Fig 2A, boxed sequence).
RT-PCR analyses, described herein and also by others, show that these eleven genes bearing the putative element are induced concurrent with excystation of parasite oocysts via heat shock.
To test whether the identified forkhead element was a functional regulator of the promoter and whether it could explain the increased stimulation of PII −516bp-luciferase by the FOXL2 C134W (Fig. 2) we generated a mutant version of the reporter, in which the putative element was mutated in the same manner as in the EMSA probes above.
The process was repeated until the full-length sequence of each putative element was obtained.
Only one putative element, weakly similar to the 3' part of a non-LTR retrotransposon in Arabidopsis (AAB82639), falls into the class I retrotransposon group.
Similar(46)
Recovered sequences were then aligned using dialign 2 (ref. 39), with the resulting output examined for the presence of possible boundaries between putative elements and the possible presence of target site duplications.
We identified an additional two putative elements in addition to the CHOP element (Fig. 2B) and named the first element MURE1 (Mitochondrial Unfolded Protein Response Element 1).
The putative promoter region of 22 genes had putative elements for 4 transcription factors, namely C/EBPalpha, NF1, Oct-1, and SP-1.
Current regulatory element prediction tools rely heavily upon conservation for prediction of putative elements.
Some of these putative elements showed a unique occurrence pattern among genes responsive to nematode infection.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com