Putative metabolic derivatives of ET – (b) hercynine, (c) ET sulfonate (ET-SO3H), and (d) S-methyl ET. ET has been found at high levels in many foods, but levels are especially high in mushrooms where it can be synthesized16,17,18.
g, Intensity distributions in laser desorption ionization Fourier-transform ion cyclotron resonance mass spectrometry (LDI-FT-ICR-MS) imaging and structures for putative xanthommatin derivatives (merged [M + H]+; [M + Na]+): decarboxylated, oxidized (m/z 380.0886; 402.0696), decarboxylated, reduced (m/z 382.1037; 404.0853), oxidized (m/z 424.0785; 446.0629) and reduced (m/z 426.0938; 426.0938).
Putative cured derivatives were examined by PCR with phage-specific primers to confirm prophage loss.
These putative haploid derivatives arose by a second, single homologous recombination (disintegration) event within the merodiploid, segregating the hupSL alleles.
An analysis of the distribution of 30 putative peptide derivatives was performed on brain sections of treated mice.
Such sequences mostly represented parasitic species (among others Encephalitozoon, Giardia, Spironucleus), which showed tendency to be positioned at the bottom of trees, including all characterized and putative CenH3 derivatives.
The second putative PMU derivative (positions 282,792 to 286,743) is much more reduced and shares only four ORFs with the larger strain AT PMU derivative.
From the fermentation of S. lasaliensis ACP12 (S970A) in the presence of the N-pentynoyl 2 -methyl hexanoate substrate 9, the novel putative polyether derivative 31 was obtained and characterized by HR-MS (Table 1, entry 13).
Putative iso-lasalocid derivatives (29– 30) were also selectively obtained when the compounds 6 and 8 were fed to S. lasaliensis ΔlasB-ACP12 (S970A) 11b (entries 11 and 12).
In the ethyl acetate extracts of the ACP12 (S970A) mutant, grown in the presence of the methyl ester substrates 3– 8 (supplemented daily to reach a 2.5 4.0 m m final concentration), the putative lasalocid A derivatives 2 and 24– 28 were identified as the major polyketide products off-loaded from the lasA PKS by micro-LC-HR-MS analyses (Table 1, entries 1 7).
