A total of 354 putative SNPs (average 25.8 Kbp apart) were predicted in the 8 cM QTL region, we selected 250 SNPs with at least 10 kb intervals for validation, The flanking sequences derived from the 6.5x scaffold sequence were used for primer design, generating 400-500 bp amplicons for resequencing by Sanger method.
From 45 Cx45-eGFP cells analysed, the average putative contact density was calculated as 23.6 ± 2.4 appositions per 100 μm.
However, it is interesting to note that this weak effect is enough to bring pit depths below the putative threshold (on average) of about 8 μm for trench formation.
Among the 587 such sequences, 232 (40%) had best hits to putative " H. walsbyi" (average aa. identity of 61%) or other haloarchaeal genes, 51 (9%) were similar to other archaea or bacteria, and another 304 (52%) had no hit in public databases.
Following lentiviral transduction, GFP was expressed in the growing putative oocytes, which averaged approximately 30 to 35 µm in size and were located at the center of aggregates.
In comparison with plant pathogens, H. minnesotensis has much fewer putative cutinases (2 vs. average 10) and pectin lyases (2 vs. average 10) (table 2) that are known to be virulence factors for plant pathogens (Xiao et al. 2012).
This can be attributed to the fact that ConCavity predicts, on average, less putative pockets than Fpocket (see Table 1).
On average 297 putative novel non-synonymous alleles were identified per individual (Table 2).
On average, one putative SNP was found every 483 bp.
Each putative spectrum had an average of six putative peptide matches and 921 putative spectra matched to one putative glycopeptide.
