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To pursue identification of a distinct marker, this study profiled the secretory proteome in 16 PDAC, 2 carcinoma in situ (CIS) and 7 benign patients using label-free mass spectrometry coupled to 1D-SDS-PAGE and Strong Cation-Exchange Chromatography (SCX).
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The DNA-binding transcription factor PAX6 was cloned 25 years ago by multiple teams pursuing identification of human and mouse eye disease causing genes, cloning vertebrate homologues of pattern-forming regulatory genes identified in Drosophila, or abundant eye-specific transcripts.
However, most analysis software are not pursuing identification of multiple true peptides.
In this study, we propose a tree-based tag identification protocol, called k-ary Tree-based Anti-collision Scheme (k-TAS) to pursue better identification efficiency.
This study was designed to pursue the identification of optimal restriction enzyme for pulsed-field gel electrophoresis (PFGE) analysis of Candida tropicalis.
Ongoing studies in our laboratory pursue the identification of additional partners of CFTR.
It is thus important to pursue the identification, cloning, and characterization of these pollen surface allergens, and this is the subject of another manuscript (in preparation).
The success of the pilot study we have presented herein will allow us to pursue further identification of models predictive of clinical cancer survival and recurrence.
This analysis provides the scientific community with additional confidence to pursue the identification of causal genetic polymorphisms underlying many complex production traits.
As the variants detected by GWASs have typically explained only a minor proportion of the heritability of complex diseases, it may prove more informative to pursue the identification and association of rare variants with disease [ 60].
While validation can be very laborious, the increased efficiency of the mapping and cloning steps opens up opportunities to pursue the identification of multiple independent alleles for the same locus.
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CEO of Professional Science Editing for Scientists @ prosciediting.com