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The initial steps include fixation that cross-links the chromatin, followed by, for most purposes, restriction enzyme digestion.
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For cloning purposes, the restriction sites XhoI and BamHI were added to the 5'- and 3'- primer, respectively.
The situation is so "desperate," Mr. Boyd said, that he, as part of a subcommittee of the Planning and Zoning Commission, is working on new zoning regulations that would grant property owners relief from setback, lot coverage and use restrictions for the purpose of historic or architectural preservation.
We concomitantly delivered a chin cup for the purpose of restriction of mandibular growth with full-time orthopedic force of 400 g per side.
For this purpose artificial restriction enzyme sites (either SfiI and NotI or EcoRI and Kpn2I) were introduced by PCR.
This study was exempt from a full review by the Institutional Review Board of Changhua Christian Hospital (permission code: 121007) because the data set consisted of de-identified secondary data that were released for research purposes without restrictions.
The federal grant had ambitious purpose, few restrictions and a not-so-distant expiration date: three years.
Meta-Analyst is made readily available to any scientist wishing to use it for non-commercial purposes, without any restriction (including the need for a material transfer agreement).
It is hard to imagine a situation where blanket restrictions on the exchange of information would constitute the "least restrictive manner" for the government to fulfil the purpose of the restriction, whatever this might be.
HARTFORD (Reuters) — The Connecticut Senate passed a bill on Saturday legalizing the use of marijuana for medical purposes, with tight restrictions intended to avoid the problems that have plagued some of the other states where it is now legal.
In s statement, the European leaders said measures should focus on trade, including goods that can be used for civilian and military purposes "and further restrictions on trade insurance".
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CEO of Professional Science Editing for Scientists @ prosciediting.com