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For this purpose, strains were cultivated on an orbital shaker in an Erlenmeyer's flasks containing 100 mL of the culture medium (potato dextrose broth), at 30 °C and 200 rpm of agitation.
For this purpose strains were grown overnight and inoculated into various fresh media.
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For that purpose, strain gauges are utilized to monitor strain concentrations near the cracks, and finite element simulations are carried out to predict the corresponding stress intensity factor distributions.
For inoculation purposes, strains were grown in Penassay broth (Difco, Md., USA).
Thus, the original small multi-purpose strain of Charolais gave way to a type of small draft horse that was next crossed with purebred and crossbred Thoroughbreds until, by 1850, it had become a horse for cavalry purposes.
For control purposes, strain DH5α/pKT1 was tested in parallel.
For this purpose, vaccine strains IVR-142 (A/Wisconsin/67/05-like A/Wisconsin/67/05-like [H3N2]onia/20/99-like [H1N1]), B/MA/Newia/2506/04, and B/Florida/4/06 were used to represent the epidemiCaledonia/20/99-like Caledonia/20/99-like [H1N1]tudy period (29 ).
For this purpose, seven strains, each of which has at least one of these three genes deleted, were constructed and characterized.
For that purpose, the strains were inoculated in 1 mL YPX medium at an initial OD600 of 1.0.
For this purpose, the strains were rendered His3+ by transformation to histidine prototrophy with a HIS3 fragment.
For that purpose, yeast strains expressing chromosomally tagged Nhp6Ap-GFP (Invitrogen) and transformed with pESC-Ura/ PEP4 were aged in SCG lacking uracil.
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