We discuss the design, operation, and calibration of two versions of a xenon gas purity monitor (GPM) developed for the EXO double beta decay program.
DNA melts assess sample purity by monitoring the SYBR® Green fluorescence signal as the temperature gradually increased from 35°C to 95°C in 60 15 second cycles where the temperature increased by 1 degree each cycle.
DNA concentration and purity were monitored on 1% agarose gels.
The phase purity, as monitored by Electron Probe Micro Analysis (EPMA) is in good agreement with the XRD data.
Radiolabeling efficiency and radio-chemical purity were monitored by thin-layer chromatography silica-gel-based thin-layer chromatography with 0.9% NaCl as developing solvent: R f of 125I-cRGD-GNPs is 0 and R f of I-125 is 1.
Labelling and final radiochemical purity were monitored by TLC in 60 mM citrate buffer, pH 5.5 (iTLCSA, Rf protein = 0, Rf [99mTc(CO)3]+ = 1) and analytical HPLC SEC (BioSep SEC-s2000, Phenomenex).
Protein purity was monitored by coomassie staining of SDS-PAGE gels.
Purity was monitored by flow cytometry with anti-CD19 mAb (eBioscience).
Spermatogonia suspension was recovered and the purity was monitored morphologically after Giemsa staining, and immunocytochemically, as described previously [26].
Undifferentiated Kit-negative spermatogonia obtained from testes of 4dpp mice, were purified by depletion of Kit-positive cells with immunomagnetic beads and their purity was monitored by FACS analysis (Fig. 3A).
RNA concentration and purity were monitored using a NanoDrop ND-1000 UV spectrophotometer (Nanodrop Technologies, Wilmington, DE).
