A prominent partial fragment of DKC1 co-purifies extensively with the full length DKC1 complexes.
Purification of the recombinant His-tagged proteins was achieved in a single step by Ni2+-charged column chromatography while proteins without His-tag were purified by extensively washing the inclusion bodies, the final yields being ∼210 and 75 mg/L, respectively.
In this study, recombinant BLyS/APRIL heterotrimers were produced by using a novel trimerization domain, purified, and extensively characterized.
This methodology is based upon the ability of mononuclear cells to spontaneously form large cellular aggregates at 4°C and has been used extensively to purify human monocytes [7], [56], [57].
Flow cytometry has been used extensively to purify populations of cells based on immunostaining, but has been mostly limited to cell surface antigens.
The process worked even with samples that had not been extensively purified, though the researchers did not attempt to directly test samples of fluids such as plasma.
We also compare the bioluminescent properties under a strictly controlled in vitro condition of selected GLuc's variants using extensively purified proteins with native disulfide bonds.
Studies with extensively purified preparations demonstrated that the agent contains a protein that is required for infectivity.
Calcium/calmodulin-dependent multifunctional protein kinases, extensively purified from rat brain (with apparent molecular mass 640 kDa), rabbit liver (300 kDa) and rabbit skeletal muscle (700 kDa), were analysed for their structural, immunological, and enzymatic properties.
Polyclonal antiserum was extensively purified by affinity chromatography.
EPS I was extracted from R. solanacearum strain UW551 and extensively purified using a modification of a described protocol [9].
