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The three reference plasmids used in this study were isolated from a purified strain of K. pneumoniae.
To evaluate this, the stable consortium N3 and the purified strain C. celevecrescens N3-2 were co-cultured with C. acetobutylicum ATCC 824, respectively.
In this study, an enriched stable consortium N3 and a purified strain Clostridium celevecrescens N3-2, which exhibited high activity of cellulose degradation at 35 °C, were firstly obtained by multiple subcultures from cellulose medium.
The high level of metronidazole resistance in this purified strain was unstable and decreased to 2 mg/L, which may be due to the absence of continual antibiotic selection.
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Four purified strains grow well individually in MS medium supplemented with 2% YE, suggesting that ZS1 growth is antagonized by other strains.
Microscopic images of purified algal strains are presented in Figure 1. Figure 1 Differential interference contrast (DIC) images of isolated unimicroalgal species (A) Scenedesmus quadricauda (B) Scenedesmus acuminatus (C) Nannochloropsis sp. (D) Anabaena sp. (E) Chlorella sp. and (F) Oscillatoria sp. The purified strains were cultivated for the autotrophic biomass production.
These experiments confirmed that the purified strains were homokaryons.
All purified strains were kept at −70°C until use.
In addition, commercially purified BoNT/A1 (strain Hall) and BoNT/A2 (strain FRI-honey) complex toxins from Metabiologics (Madison, WI) were used for comparative testing.
Then a biochemist who had isolated and purified a strain of botulinum toxin for potential military use as a biological weapon sent Dr. Scott a sample.
The recombinant enzymes had high substrate specificity toward 2-oxoglutarate as in the case of the native enzyme purified from strain TK-6.
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