Sentence examples for purified out from inspiring English sources

G protein-coupled receptors (GPCRs) are one of the most important target classes in the central nervous system (CNS) drug discovery, however the fact they are integral membrane proteins and are unstable when purified out of the cell precludes them from a wide range of structural and biophysical techniques that are used for soluble proteins.

When things hide they can't be purified out of our minds, our hearts, and our society.

"If you purify out smoking and dog . . .

"She looked up over her knitting and met the third stroke and it seemed to her like her own eyes meeting her own eyes, searching as she alone could search into her mind and her heart, purifying out of existence that lie, any lie.

With reverse osmosis systems, for example, about 80% of the water that could be purified goes out into the sewer.

Sheared samples were purified, run out on a 1.0% gel and DNA smears 200 800 bp (200 1200 bp for the E. coli samples) were isolated and purified.

Then, 50 μL of the resuspended bacteria was added to a 2 mM electroporation cuvette along with 15 μL of purified knock-out cassette.

Although not explored, it seems likely that the preparation consisted of purified inside-out membranes containing the Cx43 fusion protein because detergents and centrifugation procedures to separate membranes from soluble proteins were not used [ 29].

PCR products were electrophoresed in 2% agarose gels using 1x TAE buffer (40 mM TRIS, 20 mM sodium acetate, and 1 mM EDTA adjusted to pH 7.2 with glacial acetic acid) with ethidium bromide and purified (Gel-Out Kit, A&A Biotechnology, Poland) and cloned into the pJet 1.2 vector (CloneJET PCR Cloning Kit, Thermo Scientific, USA) in accordance with the manufacturers protocols.

PCR products for Olfml1 and Riok2 were cut out from the gel, purified and sent out for sequencing to Makrogen.

Because studies have reported negative effects for plant cystatins against a number of pathogenic fungi including B. cinerea (e.g. [ 48- 52]), in vitro bioassays were carried out with purified CCII to rule out a possible, although unexpected, protease inhibition-independent toxic effect against the model fungus.