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A semi-automated solid-phase extraction method was used to prepare purified extracts for AhR-mediated transcriptional activity analyses from a single 5-ml plasma sample as described in Medehouenou et al. [ 54].
Partially purified extracts from N. fowleri showed the coexistence of glutathione and trypanothione reductases activities.
Partially purified extracts from the leaves of transgenic tobacco plants inhibited in vitro growth progression of Plasmodium falciparum-infected red blood cells.
Detection of N. fuckeliana from bark preparations was only possible following nested PCR of PVPP purified extracts using universal primers ITS5 and 4 in first round amplification.
While the non-catalytic supercritical methanol method produces highly purified extracts (free of harmful solvents and catalyst residues), and reduces energy consumption in separation and purification steps.
The line's Baume 27, a thick pomade that aims to rebalance aging skin, also has three purified extracts of Centella asiatica, which Ms. Evrard believes has "repairing properties".
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Re-chromatography of these semi-purified extracts on silica gel eluting with ethyl acetate-isopropanol-ammonia (85:14:1) yielded cyclopamine pure enough to solidify by trituration with cold acetone.
Subsamples of the gel-purified extracts were therefore added to cloning reactions (TOPO TA cloning kit, Invitrogen).
Among the un-purified extracts we found seven agonists for the EP1 receptors.
In this study we investigated the effect of semi-purified extracts of C. benghalensis on growth-associated molecular events of apoptosis and cell division cycle of JT cells.
No significant differences were obtained for the CCα between the crude extracts and the SPE-purified extracts, except for YTX and hydrolysed OA.
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