Sentence examples for purified elementary from inspiring English sources

The purified elementary bodies were not further treated (e.g. by removal of lipopolysaccharide).

Purified elementary bodies were pelleted, washed, resuspended in sucrose-phosphate-glutamate buffer, and stored in aliquots at -70°C.

The sensitivity of the PCR was evaluated by testing 10-fold serial dilutions of DNA extracted from purified elementary bodies of Cp. psittaci strains 92/1293 [ 2].

Since both the SeroCP test and our MIF test use purified elementary bodies of Cpn as antigen, both assays measure antibodies directed against antigens localized on the surface of Cpn elementary bodies.

Cpn species specific IgG and IgA antibodies were measured by our in house MIF test as described by Wang and Grayston [ 17] using as antigens purified elementary bodies of Cpn, IOL-207 strain, Chlamydophila psittaci Loth strain and Chlamydia trachomatis (Ct) L2 strain.

Amplification conditions were highly optimized as the chlamydial DNA was prepared from semi-purified elementary bodies: the approach in the Moscow study is therefore not applicable to routine clinical samples.

Chlamydia elementary bodies were harvested, purified and quantified [15] and stored at −80°C in a sucrose-phosphate-glutamate (SPG) buffer.

The elementary bodies (EB) were purified on Renograffin gradients, aliquoted in sucrose-phosphate-glutamine buffer and stored at −80°C as described [28].

Infectious elementary bodies (EBs) were purified by renografin (Squibb diagnostics, New Bronswick, NJ) density gradient centrifugation; alternatively lysates from infected cells were used to infect monolayers.

Elementary bodies (EBs) were harvested and purified by disruption of HEp-2 cell monolayers with a cell scraper, sonication and centrifugation over a renografin density gradient [ 28].

Guilty pleasures were purified.