Briefly, the two sheets of infected ear dermis were separated, deposited in DMEM containing 100 U/ml penicillin, 100 μg/ml streptomycin and 0.2 mg/ml Liberase CI purified enzyme blend (Roche Diagnostics Corp., Chicago, IL, USA) and incubated for 1 h and 30 min at 37 °C.
The pea protein is further purified and blended together with water and other ingredients, including sunflower oil and sea salt, as well as vitamins like B12.
Fully infiltrated tissues were embedded in Histowax (highly purified paraffin wax blended with polymer additives).
In Figure 1, curves for the A. cellulolyticus, A. awamori, A. cellulolyticus- A. awamori blend, partially purified β-glucosidase, and the control were best fitted into a mono-exponential model, and the curves for T. reesei and the T. reesei- A. awamori blend were best fitted into a double exponential model.
Enzymatic treatment of the dissected tissue prior to dissociation was done with a commercial blend of purified collagenase I, collagenase II and the neutral protease dispase (Liberase Blendzyme 1, #1988409; Roche, Indianapolis, IN) at a concentration of 72 μg/ml in Rinaldini's saline.
By raw I will reference a favorite online dictionary: - Being in or nearly in the natural state : not processed or purified : not diluted or blended - Unprepared or imperfectly prepared for use - Not being in polished, finished or processed form.
The purified HAs are then blended and filled into single-dose vials [ 67, 68].
Chloroplasts were released from purified BS fibres by blending on high setting, in a small Waring blender with the blades replaced with a razor blade, in 30 second pulses until the grinding buffer turned green.
Four separate aliquots from the BCR-027 Zn blend digest were purified and analyzed over the course of three separate analytical sessions.
These are purified from human whole blood, blended together, and used to calibrate the primary reference measurement system.
The treated effluent would be further purified using advanced treatment, then blended with reservoir water at a 50/50 ratio and treated once again.
