Sentence examples for purification trials from inspiring English sources

Expression and purification trials of cloned targets identified the protein MCMJR1 from the euryarchaeon Methanoculleus marisnigri JR1, as an optimal expressor that was stable during purification in detergent.

Our initial F420 purification trials indicated that M. smegmatis transformed to over express the M. tuberculosis protein FGD1 (F420–dependent glucose-6-phosphate dehydrogenase 1) could produce higher levels of F420 compared with the wild type strains.

In addition, despite their low extraction efficiency, LDAO and DDM were also included in our purification trials because they have been successfully used for purifying and characterizing other ABC transporters [32], [33], and are among the preferred detergents for crystallization of membrane proteins [34].

As structural studies of hOR17-4 would not only provide information crucial to understanding the molecular mechanism(s) of olfaction but also have application to human reproduction and population control, we thus selected this receptor as our prototype OR for expression and purification trials.

In our initial purification trials we focused on optimising recovery of PB material from the mycelia.

Initial purification trials of the recombinant PNGase H+ using Tris/HCl (pH 8.0) as buffer yielded only small amounts of purified protein after nickel affinity chromatography.

The eight DNA extraction and purification protocols trialled are described below, with the key differences between the methods outlined in Table 1.

Talon beads, which employ Cobalt to chelate the His-tag, were used for the affinity purification as trial experiments proved them better than Ni-based beads (data not shown).

Several rounds of protein purification and crystallization trials yielded tiny needle clusters that could not be improved in size or quality.

To facilitate purification, different expression trials (Electronic supplementary material) were conducted to increase the protein production in the soluble form.

In stage 1, eight alternative DNA extraction and purification protocols were trialled using pine needle tissue, using the standard tissue input recommendations for each method.