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S.T. and A.B. designed the purification strategy for the PLC.
This purification strategy was successfully demonstrated for all 9 IgMs in the panel.
Purification strategy was designed, considering of the compound enrichment, sample purity and purification throughput.
As a purification strategy, an electrocoagulation (EC) process followed by a biological purification step was applied.
A rapid and simple purification strategy was established by incorporating a FLAG-tag at the C-terminus of the protein.
A detailed purification strategy for thaumatin is reported resulting in a homogenous sample recovered at a yield of 42%.
This two-step purification strategy resulted in a Murl sample with a specific activity of 34,060 nkatd-Glu/mgprotein, comprising a single protein band in SDS PAGE.
Through a tandem affinity purification strategy and mass spectrometry, we depicted an interactomic landscape of major members of the mammalian Rab GTPase family.
Here we present a solution that employs an orthogonal purification strategy to achieve the quantity and level of purity necessary for further studies of this complex.
However, the development of the effective purification strategy remains the major challenge for large-scale production of therapeutic proteins because high purity of the proteins is often demanded.
An alternate purification strategy yields highly pure DHFR complexes, containing only the desired ligands, in the quantities required for structural studies.
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CEO of Professional Science Editing for Scientists @ prosciediting.com