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This purification step was repeated once.
This allows a low-energy intermediate purification step [2].
(A) The first purification step used Superdex 200.
a The first purification step used Phenyl Sepharose CL-4B.
Activated alumina was used as a second purification step.
(B) The second purification step used immobilized D-galactose.
b The second purification step used Concanavalin A-Sepharose 4B.
Virtually pure proteins were obtained in a single purification step.
IMAC is the first purification step to enrich the NTS1 fusion protein.
A second purification step was performed with RNeasy columns (Qiagen, Hilden, Germany).
STEP 2 - Different gradients were used for this second purification step.
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