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In order to solve its purification problem, we performed the surface potential analysis with GRASP2 program.
However, we failed to utilize TAT-ZFN to modify the endogenous gene because of a purification problem.
The phage purification problem is growing with the new interest in phage therapy that results from the crisis of antibiotic resistance in bacteria.
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Very frequently filter elements made from pleated filter media are used to solve gas purification problems.
Since the chains lengthen in a stepwise manner incomplete reactions cause impurities which decrease synthetic yield and pose significant purification problems.
Design and optimal operation of an enzymatic process depend on the characteristics of the free- vs. immobilized-enzyme, purification problems, materials and operation costs.
So, water purification problems become more and more important.
The additional amino acids derived from GATEWAY tags may interfere with subsequent experiments, such as soluble expression of the recombinant protein, purification problems due to aggregation of the protein, and crystallization of the protein (see descriptions in Background).
This last deprotection step turned out to be the bottleneck of this synthetic strategy, leading to low yields (≈20 50 %) due to the sensitivity of the electron-rich oligo phenylene ethynylene) core under acidic conditions, the long reaction time required, and the incomplete deprotection of both glycerol groups, giving rise to purification problems.
Mucolytic agent and filter purification (DTT) – One problem that occurs when using large quantities of intestinal contents for phage isolation is interference of intestinal mucus during filtration.
It is concluded that reliance on landscape metabolism and self-purification postpones the problem of global N overload and partially transfers it to the atmosphere.
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