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After centrifugation at 1000 x g for 5 min, the chloroform containing the extracted sterigmatocystin was transferred to a fresh tube, washed twice with 0.5 mL Milli-Q water (QPAK 2 purification pack, Millipore) and then evaporated.
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The solvent was evaporated, followed by silica-gel packed column chromatographic purification using ethyl acetate : hexane (4 : 1, v/v) as an eluent to yield 5-hydroxytryptophan methyl ester hydrochloride 5.18 g (84% yield).
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