Sentence examples for purification of targets from inspiring English sources
Using a modification of a published procedure [ 23], we designed and synthesized an isothiocyanate molecule that contained a biotin moiety, termed Bio-ITC, to enable specific detection and potential affinity purification of targets of covalent ITC modification.
Figure 4: Solubility analysis of fusion proteins and subsequent purification of target peptides and protein.
In this case, a separation and a purification of target product become the important steps of the whole process.
Heat treatment is often used during the processing of plant-derived biomass to precipitate host cell proteins (HCPs) and thus simplify the purification of target proteins.
The HB-tag has also been shown to facilitate the purification of target recombinant proteins from their 8 M urea denatured state(s).
Fast dissociation kinetics may be, however, limiting for using Strep-tag®II for direct purification of target proteins from large volumes of diluted extracts like mammalian cell culture supernatants or in assay formats requiring extended washing like ELISA.
Since the binding of HaloTag to the HaloLink resin is essentially irreversible, it overcomes the equilibrium-based binding limitations associated with affinity tags and enables efficient capture and purification of target protein, even at low expression levels.
The benefits of plants for the production of biopharmaceutical proteins include the ease of scale-up and the low pathogen burden, but the purification of target proteins is often affected by low expression levels and the abundance of host cell proteins in leaf extracts.
The target based screening approach involves purification of target protein, further compound screening assays against the target and in vivo target validation.
For a long time, purification of target enzymes from wild-type strains has been the dominant approach for enzyme discovery [41].
The accumulation of these by-products often inhibits the growth of cells, competes for NADH against the 1,3-PD pathway to reduce the yield of 1,3-PD from glycerol, and brings difficulties for the separation and purification of target product (Xiu and Zeng 2008).
