Sentence examples for purification of target from inspiring English sources
Figure 4: Solubility analysis of fusion proteins and subsequent purification of target peptides and protein.
In this case, a separation and a purification of target product become the important steps of the whole process.
Heat treatment is often used during the processing of plant-derived biomass to precipitate host cell proteins (HCPs) and thus simplify the purification of target proteins.
The HB-tag has also been shown to facilitate the purification of target recombinant proteins from their 8 M urea denatured state(s).
Fast dissociation kinetics may be, however, limiting for using Strep-tag®II for direct purification of target proteins from large volumes of diluted extracts like mammalian cell culture supernatants or in assay formats requiring extended washing like ELISA.
Since the binding of HaloTag to the HaloLink resin is essentially irreversible, it overcomes the equilibrium-based binding limitations associated with affinity tags and enables efficient capture and purification of target protein, even at low expression levels.
Using a modification of a published procedure [ 23], we designed and synthesized an isothiocyanate molecule that contained a biotin moiety, termed Bio-ITC, to enable specific detection and potential affinity purification of targets of covalent ITC modification.
In the present study, we observed that the aptamer without a linker showed the same capacity to pick up the target as the aptamer with a linker, indicating that very minimal modification is needed for the successful purification of the target by an affinity pull down assay (Fig. S1).
pTYB1 uses ATG of the NdeI site in the multiple cloning region for translation initiation and contains a SapI cloning site, which allows the target gene to be cloned adjacent to the cleavage site of the intein tag; this results in the purification of a target protein without any non-native residues.
NT* allows for efficient purification of hydrophobic target peptides using just simple NaCl precipitation and ethanol extraction step since none of the CNBr-cleaved fragments of NT* dissolve in the ethanol fraction.
In some cases, purification of unknown target molecules is facilitated by the ability to cleave the cross-linking bonds after isolation of the complex.
