Details of expression, purification, crystallization, and structure determination are in the 'Materials and methods'.
Expression and Purification of OTULIN OTULIN was expressed in E. coli strain Rosetta2 (DE3) pLacI.
Purification and expression of genetically engineered CaM with cysteine on N-terminus is based on instructional manual prepared by New England Biolabs [20].
The experimental conditions of expression and purification of each recombinant protein were optimized.
Induction of expression and purification of recombinant His-tagged WRKY25 protein were performed according to the protocol provided by Novagen.
An assay to follow the progress of expression and purification of functional protein is an absolute key to study VGSCs.
This provides evidence of the fidelity of expression and purification of post-translationally modified human type II procollagen from our system.
It used to be as a common method for the purification of rHLZ in other expression systems [29], [39].
Construction, expression, and purification of site-directed mutant proteins of CcP were performed as described previously.
Construction, expression, and purification of the Y254V mutant has been described.
